Proclinical is seeking a an innovative Scientist with a background in RNA Molecular Biology to join a well funded Biotech in Cambridge, MA newly founded to make the world's first programmable RNA medicines. With a rich product pipeline that covers multiple therapeutic areas and is in various stage of development, we are well funded, with an experienced management team, and world class Scientific Advisory Board (SAB) members.
As a Senior/ Principal Scientist in the RNA biology group, you will be a key contributor to a diverse team working on novel siRNA modalities as well as in vitro assays and in vivo measurements of drug activity. The ideal candidate will have experience of experience of siRNA/Oligotherapeutics, molecular biology, cell biology and in vivo assays. You will be supporting the development of the next generation of RNA drugs and ensuring that the current generation reach the patients that need them. We are looking for innovators, thinkers and doers.
Responsibilities:
- Be one of the first members of the biology team, helping to shape its culture and dynamic
- Perform experiments within the team: Execution, optimization and understanding of protocols for protein chemistry and target validation, such as RT-PCR, SDS-PAGE, ELISA, western blots
- Proven expertise in RNAi space
- Clearly document experimental workflows and results; Communicate findings to the team and management when required
- Strong background in molecular biology and RNA metabolism regulation; cancer biology background is preferred.
- In vivo experience; cancer biology in vivo background is preferred
- Organize/assembly appropriate materials, independently design, supervise and execute advance principles of molecular cloning for expression vectors (Gateway, Gibson assembly, Lentiviral & Adenoviral vectors generation)
- Supervise and execute viral particles generation and viral transduction of mammalian cells.
- Oversee day to day lab tasks
- Independently design, perform and/or supervise in vitro siRNA cytotoxicity cell-based assays.
- Independently search the literature for experiment and research design along with Senior/Principal Scientist.
- Development, optimization, and troubleshooting of methods to screen and characterize activity of siRNA/RNA/antisense oligonucleotides (ASOs)
- Development and optimization of in vitro RNA techniques (biotin RNA pull downs, RNA-IP, CLIP assays)
- Research and development of in vitro assays to evaluate impact of siRNA/antisense oligonucleotides on RNA transcripts, protein expression, and other project-specific assays
- Apply molecular and cell-based assays including but not limited to RNA transfection, RNA-silencing, RNA & protein isolation, gene expression analysis (RT-qPCR), western blot, and fluorescence immunocytochemistry
- Understanding and experience in performing genome-wide transcriptomic assays (RNA-sequencing)
- Clearly document experiments, organize and communicate data with scientific supervisors.
Supervise and mentor Research associates within the Biology team.
Requirements:
- PhD degree: relevant life sciences discipline preferred
- Extensive industry research experience
- Familiarity with siRNA, ASOs and gene silencing
- Ability to adapt to change and thrive in a dynamic and entrepreneurial early-stage environment.
If you are having difficulty in applying or if you have any questions, please contact Max Robinson at (+1) 857-362-9542 or M.Robinson@proclinical.com.
Proclinical is a specialist employment agency and recruitment business, providing job opportunities within major pharmaceutical, biopharmaceutical, biotechnology and medical device companies.
Proclinical Staffing is an equal opportunity employer.
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