Scientist in Oligonucleotide Biology job in Cambridge, USA

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Last Name*

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Phone Number*

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Are you an employer or a jobseeker?*

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First Name*

Last Name*

Email*

Phone Number*

Country*

Are you an employer or a jobseeker?*

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First Name*

Last Name*

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Phone Number*

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Job type: Permanent

Discipline: Biochemistry, Biological Sciences, Laboratory Technician

Location: United States

This vacancy has now expired. Please see similar roles below...

Proclinical is seeking to hire a Scientist in Oligonucleotide Biology located in Cambridge, MA. This individual will work with an innovative, dynamic and visionary team creating programmable medicines.

Job Responsibilities:

Perform experiments within the biology team: Execution, optimisation and understanding of protocols for protein chemistry and target validation, such as RT-PCR, SDS-PAGE, ELISA, western blots.
Clearly document experimental workflows and results; Communicate findings to the team and management when required.
Organise/assembly appropriate materials, independently design, supervise and execute advance principles of molecular cloning for expression vectors (Gateway, Gibson assembly, Lentiviral & Adenoviral vectors generation).
Supervise and execute viral particles generation and viral transduction of mammalian cells.
Oversee day to day lab tasks.
Independently design, perform and/or supervise in vitro siRNA cytotoxicity cell-based assays.
Independently search the literature for experiment and research design along with Senior/Principal Scientist.
Development, optimisation, and troubleshooting of methods to screen and characterise activity of siRNA/RNA/antisense oligonucleotides (ASOs).
Development and optimisation of in vitro RNA techniques (biotin RNA pull downs, RNA-IP, CLIP assays).
Research and development of in vitro assays to evaluate impact of siRNA/antisense oligonucleotides on RNA transcripts, protein expression, and other project-specific assays.
Apply molecular and cell-based assays including but not limited to RNA transfection, RNA-silencing, RNA & protein isolation, gene expression analysis (RT-qPCR), western blot, and fluorescence immunocytochemistry.
Understanding and experience in performing genome-wide transcriptomic assays (RNA-sequencing)

If you are having difficulty in applying or if you have any questions, please contact Danielle Bahnasy at (+1) 646-868-5142or d.bahnasy@proclinical.com.

Proclinical is a specialist employment agency and recruitment business, providing job opportunities within major pharmaceutical, biopharmaceutical, biotechnology and medical device companies.

Proclinical Staffing is an equal opportunity employer.

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