Scientist, Oligonucleotide Biology job in Cambridge, USA

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Last Name*

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Job type: Permanent

Discipline: Biochemistry, Biological Sciences, Bioprocessing

Location: United States

This vacancy has now expired. Please see similar roles below...

Proclinical is seeking to hire a Scientist, Oligonucleotide Biology located in Cambridge, MA. This individual will join an innovative, dynamic and visionary team creating programmable medicines.

Job Responsibilities:

Perform experiments within the biology team: Execution, optimization and understanding of protocols for protein chemistry and target validation, such as RT-PCR, SDS-PAGE, ELISA, western blots
Clearly document experimental workflows and results; Communicate findings to the team and management when required
Organize/assembly appropriate materials, independently design, supervise and execute advance principles of molecular cloning for expression vectors (Gateway, Gibson assembly, Lentiviral & Adenoviral vectors generation)
Supervise and execute viral particles generation and viral transduction of mammalian cells.
Oversee day to day lab tasks
Independently design, perform and/or supervise in vitro siRNA cytotoxicity cell-based assays.
Independently search the literature for experiment and research design along with Senior/Principal Scientist.
Development, optimization, and troubleshooting of methods to screen and characterize activity of siRNA/RNA/antisense oligonucleotides (ASOs)
Development and optimization of in vitro RNA techniques (biotin RNA pull downs, RNA-IP, CLIP assays)
Research and development of in vitro assays to evaluate impact of siRNA/antisense oligonucleotides on RNA transcripts, protein expression, and other project-specific assays
Apply molecular and cell-based assays including but not limited to RNA transfection, RNA-silencing, RNA & protein isolation, gene expression analysis (RT-qPCR), western blot, and fluorescence immunocytochemistry
Understanding and experience in performing genome-wide transcriptomic assays (RNA-sequencing)
Clearly document experiments, organize and communicate data with scientific supervisors.
Supervise and mentor Research associates within the Biology team

Skills and Requirements:

PhD degree within a relevant life sciences discipline
1+ years of relevant laboratory experience in academia or industry
Proven experience within the RNAi space
Strong background in molecular biology and RNA metabolism regulation; cancer biology background is preferred.
In vivo experience; cancer biology in vivo background is preferred
Familiarity with siRNA, ASOs and gene silencing
Ability to adapt to change and thrive in a dynamic and entrepreneurial early-stage environment.

If you are having difficulty in applying or if you have any questions, please contact Sofia Amark at (+1) 646-367-2764 or s.amark@proclinical.com.

Proclinical is a specialist employment agency and recruitment business, providing job opportunities within major pharmaceutical, biopharmaceutical, biotechnology and medical device companies.

Proclinical Staffing is an equal opportunity employer.

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